简要描述：BD Pharmingen 7-AAD核酸染料 559925，7-Amino-Actinomycin D (7-AAD)

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BD Pharmingen 7-AAD核酸染料 559925，7-Amino-Actinomycin D (7-AAD)

（7-AAD） 是一种即用型核酸染料，可替代碘化丙啶（PI）用于流式细胞检测中非活细胞的排除。与PI相比，7-AAD的优势在于能与藻红蛋白（PE）和异硫氰酸荧光素（FITC）标记的单克隆抗体联用，进行双色分析，且7-AAD、PE和FITC的荧光发射光谱重叠极小。7-AAD的荧光在远红光光谱范围内检测（650 nm长通滤光片）。

该试剂作为一种活力探针，基于光散射和FL3通道检测的7-AAD摄取，用于死细胞排除方法。无需稀释，建议用量为每测试（1×10^6个细胞）添加5 µl（0.25 µg），分析前孵育10分钟。

7-Amino-Actinomycin D (7-AAD) is a convenient, ready-to-use nucleic acid dye that can be used in place of propidium iodide (PI) for the exclusion of nonviable cells in flow cytometric assays. The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labelled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).  This reagent is used as a viability probe for methods of dead cell exclusion, based on light scatter and uptake of 7-AAD as detected in FL3.  This reagent does not require dilution.  Suggested quantity to use:  5 µl (0.25 µg)/test (1x10^6 cells) and incubate for 10 minutes before analysis.

BD Pharmingen 7-AAD核酸染料 559925，7-Amino-Actinomycin D (7-AAD)

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.